… Because the effects of the two individual mutations are preserved in the double mutant and
… be independently reversed, we conclude that residues Asp 96 and Leu 93 act independently …

… 4I,93-96 Because of this complication, however, studies of … However, such a hydrogen-bonding
to Asn96 cannot develop, or … Leu-93 is at nearly contact distance from the retinal chain at …

… in this double mutant than in wild-type bacteriorhodopsin (Fig… -reconstituted Leu-93 → Ala,
Asp-96 → Asn mutant was … of the photocycle of the Leu-93 mutants, we studied the effects of …

… near Leu-93 was inferred from the effects of various mutations … it, alone or in combination
with other Asp residues with Asn, … double mutant, residue 85 is neutral, and the pK a of Asp-96 …

… The L to M reaction of the bacteriorhodopsin photocycle includes the … From infrared and
resonance Raman studies it is widely … of these residues (or in the case of Asp96 perhaps even its …

… Crystal structures of the Asp 96 to Asn mutant of the light-… of the retinal during the photocycle
in mutants of Leu 93 (23) as well … Studies of the photocycle of the D85N–D96N mutant (34) …

… of which affect the photocycle kinetics and the environment of … gap between Asp-96 and the
Schiff base (Rothschild et al.. 1993). … The goal of this research is to further clarify the role of …

… carboxylic residues corresponding to Asp-85 and Asp-96 of … , similar to one described earlier
for Leu-93 mutants of BR ( 33, … KL to L in the bacteriorhodopsin photocycle. Identification of …

… and the M intermediate (89-93). The publication of these … photocycle and proton pump at
the structural level. One of the results of these studies coupled with sitedirected spin labeling (96…

… Hydrophobic residues as Trp and Leu and the barely … After reprotonation of Asp-96 from
the cytoplasmic surface (O … R., et al., Properties of Asp212----Asn bacteriorhodopsin suggest …